Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Yeast two-hybrid screening of proteins interacting with plasmin receptor subunit: C-terminal fragment of annexin A2.

Li Q, Laumonnier Y, Syrovets T, Simmet T

To identify proteins that interact with the C-terminal fragment of annexin A2 (A2IC), generated by plasmin cleavage of the plasmin receptor, a heterotetramer (AA2t) containing annexin A2.The gene that encodes the A2IC fragment was obtained from PCR-amplified cDNA isolated from human monocytes, and was ligated into the pBTM116 vector using a DNA ligation kit. The resultant plasmid (pBTM116-A2IC) was sequenced ... [more]

Acta Pharmacol. Sin. Nov. 01, 2011; 32(11);1411-8 [Pubmed: 21963895]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ANXA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

S100 protein binding [IPI]calcium-dependent phospholipid binding [IDA]calcium-dependent protein binding [IPI]phosphatidylinositol-4,5-bisphosphate binding [IMP]phospholipase A2 inhibitor activity [IDA]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

PAPD4