Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human Regulatory Protein Ki-1/57 Is a Target of SUMOylation and Affects PML Nuclear Body Formation.

Saito A, Souza EE, Costa FC, Meirelles GV, Goncalves KA, Santos MT, Bressan GC, McComb ME, Costello CE, Whelan SA, Kobarg J

Ki-1/57 is a nuclear and cytoplasmic regulatory protein first identified in malignant cells from Hodgkin's lymphoma. It is involved in gene expression regulation on both transcriptional and mRNA metabolism levels. Ki-1/57 belongs to the family of intrinsically unstructured proteins and undergoes phosphorylation by PKC and methylation by PRMT1. Previous characterization of its protein interaction profile by yeast two-hybrid screening showed ... [more]

J. Proteome Res. Dec. 01, 2016; 16(9);3147-3157 [Pubmed: 28695742]

Throughput

  • High Throughput

Additional Notes

  • SERBP1 co-expressed with Sumo1
  • SERBP1 co-expressed with Sumo2

Curated By

  • BioGRID