PTPRC
Gene Ontology Biological Process
- B cell proliferation [ISS]
- B cell receptor signaling pathway [ISS]
- T cell differentiation [ISS]
- T cell receptor signaling pathway [IDA, TAS]
- axon guidance [TAS]
- bone marrow development [IMP]
- cell cycle phase transition [IMP]
- cell surface receptor signaling pathway [TAS]
- defense response to virus [ISS]
- dephosphorylation [ISS]
- hematopoietic progenitor cell differentiation [IMP]
- immunoglobulin biosynthetic process [IMP]
- negative regulation of T cell mediated cytotoxicity [ISS]
- negative regulation of cell adhesion involved in substrate-bound cell migration [IMP]
- negative regulation of cytokine-mediated signaling pathway [ISS]
- negative regulation of protein kinase activity [IDA, ISS]
- peptidyl-tyrosine dephosphorylation [IDA, TAS]
- positive regulation of B cell proliferation [IMP]
- positive regulation of T cell proliferation [ISS]
- positive regulation of antigen receptor-mediated signaling pathway [ISS]
- positive regulation of hematopoietic stem cell migration [IMP]
- positive regulation of protein kinase activity [NAS]
- positive regulation of stem cell proliferation [IMP]
- protein dephosphorylation [ISS]
- regulation of cell cycle [ISS]
- release of sequestered calcium ion into cytosol [ISS]
- stem cell development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PTPRCAP
Gene Ontology Biological Process
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
CD45 protein-tyrosine phosphatase associates with the WW domain-containing protein, CD45AP, through the transmembrane region.
CD45 is a transmembrane protein-tyrosine phosphatase required for antigen receptor signaling in lymphocytes. CD45 activates the Src family protein-tyrosine kinases, p56lck and p59fyn, by dephosphorylating a negative regulatory tyrosine in the carboxyl terminus. Immunoprecipitation of CD45 precipitates p56lck and CD45AP. Although the function of CD45AP is unknown, it has been proposed to be an adapter between p56lck and CD45. To ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PTPRC PTPRCAP | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID