Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Phosphorylation of alpha-actinin 4 upon epidermal growth factor exposure regulates its interaction with actin.

Shao H, Wu C, Wells A

The ubiquitously expressed family of alpha-actinins bridges actin filaments to stabilize adhesions, a process disrupted during growth factor-induced migration of cells. During the dissolution of the actin cytoskeleton, actinins are phosphorylated on tyrosines, although the consequences of this are unknown. We expressed the two isoforms of human alpha-actinin in murine fibroblasts that express human epidermal growth factor receptor (EGFR) and ... [more]

J. Biol. Chem. Jan. 22, 2010; 285(4);2591-600 [Pubmed: 19920151]

Throughput

  • Low Throughput

Curated By

  • BioGRID