BAIT
DCPS
DCS1, HINT-5, HINT5, HSL1, HSPC015
decapping enzyme, scavenger
GO Process (8)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- cellular response to menadione [IDA]
- exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay [TAS]
- gene expression [TAS]
- mRNA cis splicing, via spliceosome [IDA]
- mRNA metabolic process [TAS]
- negative regulation of programmed cell death [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PRKDC
DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350
protein kinase, DNA-activated, catalytic polypeptide
GO Process (13)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular protein modification process [TAS]
- cellular response to insulin stimulus [IMP]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [IBA]
- double-strand break repair via nonhomologous end joining [TAS]
- innate immune response [TAS]
- negative regulation of protein phosphorylation [ISS]
- peptidyl-serine phosphorylation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of type I interferon production [TAS]
- regulation of circadian rhythm [ISS]
- signal transduction involved in mitotic G1 DNA damage checkpoint [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS.
To identify novel targets for acute myeloid leukemia (AML) therapy, we performed genome-wide CRISPR-Cas9 screening using AML cell lines, followed by a second screen in vivo. Here, we show that the mRNA decapping enzyme scavenger (DCPS) gene is essential for AML cell survival. The DCPS enzyme interacted with components of pre-mRNA metabolic pathways, including spliceosomes, as revealed by mass spectrometry. RG3039, a ... [more]
Cancer Cell Dec. 12, 2017; 33(3);386-400.e5 [Pubmed: 29478914]
Throughput
- High Throughput
Curated By
- BioGRID