BAIT
DCPS
DCS1, HINT-5, HINT5, HSL1, HSPC015
decapping enzyme, scavenger
GO Process (8)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- cellular response to menadione [IDA]
- exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay [TAS]
- gene expression [TAS]
- mRNA cis splicing, via spliceosome [IDA]
- mRNA metabolic process [TAS]
- negative regulation of programmed cell death [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GNAI2
GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
GO Process (11)
GO Function (5)
GO Component (9)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- adenosine receptor signaling pathway [IBA]
- adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway [IBA, TAS]
- blood coagulation [TAS]
- cell division [IMP]
- gamma-aminobutyric acid signaling pathway [IBA]
- negative regulation of adenylate cyclase activity [TAS]
- platelet activation [TAS]
- response to nutrient [TAS]
- signal transduction [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS.
To identify novel targets for acute myeloid leukemia (AML) therapy, we performed genome-wide CRISPR-Cas9 screening using AML cell lines, followed by a second screen in vivo. Here, we show that the mRNA decapping enzyme scavenger (DCPS) gene is essential for AML cell survival. The DCPS enzyme interacted with components of pre-mRNA metabolic pathways, including spliceosomes, as revealed by mass spectrometry. RG3039, a ... [more]
Cancer Cell Dec. 12, 2017; 33(3);386-400.e5 [Pubmed: 29478914]
Throughput
- High Throughput
Curated By
- BioGRID