PREY

AKT2

2410016A19Rik, AW554154, PKB, PKBbeta
thymoma viral proto-oncogene 2
GO Process (30)
GO Function (5)
GO Component (8)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Phosphoproteomics screen reveals akt isoform-specific signals linking RNA processing to lung cancer.

Sanidas I, Polytarchou C, Hatziapostolou M, Ezell SA, Kottakis F, Hu L, Guo A, Xie J, Comb MJ, Iliopoulos D, Tsichlis PN

The three Akt isoforms are functionally distinct. Here we show that their phosphoproteomes also differ, suggesting that their functional differences are due to differences in target specificity. One of the top cellular functions differentially regulated by Akt isoforms is RNA processing. IWS1, an RNA processing regulator, is phosphorylated by Akt3 and Akt1 at Ser720/Thr721. The latter is required for the recruitment ... [more]

Mol. Cell Feb. 20, 2014; 53(4);577-90 [Pubmed: 24462114]

Throughput

  • Low Throughput

Curated By

  • BioGRID