An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Real-time tracking of complex ubiquitination cascades using a fluorescent confocal on-bead assay.

Koszela J, Pham NT, Evans D, Mann S, Perez-Pi I, Shave S, Ceccarelli DFJ, Sicheri F, Tyers M, Auer M

The ubiquitin-proteasome system (UPS) controls the stability, localization and/or activity of the proteome. However, the identification and characterization of complex individual ubiquitination cascades and their modulators remains a challenge. Here, we report a broadly applicable, multiplexed, miniaturized on-bead technique for real-time monitoring of various ubiquitination-related enzymatic activities. The assay, termed UPS-confocal fluorescence nanoscanning (UPS-CONA), employs a substrate of interest immobilized ... [more]

BMC Biol. Dec. 10, 2017; 16(1);88 [Pubmed: 30097011]

Throughput

Low Throughput

Additional Notes

Source of Ube2H not clear.
Ubiquitin transthiolation.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBE2H UBA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Chrustowicz J (2024)	High	-	BioGRID	-
UBA1 UBE2H	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Huguenin-Dezot N (2016)	Low	-	BioGRID	2515831
UBA1 UBE2H	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Jin J (2007)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

UBA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]ubiquitin activating enzyme activity [IBA, IDA]ubiquitin-protein transferase activity [IBA]

Gene Ontology Cellular Component

UBE2H

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]small conjugating protein transferase activity [IBA]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [IDA]