BAIT

MKL2

MRTF-B, MRTFB, NPD001
MKL/myocardin-like 2
GO Process (2)
GO Function (2)
GO Component (1)
Homo sapiens
PREY

SRF

MCM1
serum response factor (c-fos serum response element-binding transcription factor)
GO Process (23)
GO Function (9)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Megakaryoblastic leukemia-1/2, a transcriptional co-activator of serum response factor, is required for skeletal myogenic differentiation.

Selvaraj A, Prywes R

Serum response factor (SRF) is required for the expression of a wide variety of muscle-specific genes that are expressed upon differentiation and is thus required for both striated and smooth muscle differentiation in addition to its role in regulating growth factor-inducible genes. A heart and smooth muscle-specific SRF co-activator, myocardin, has been shown to be required for cardiac development and ... [more]

J. Biol. Chem. Oct. 24, 2003; 278(43);41977-87 [Pubmed: 14565952]

Throughput

  • Low Throughput

Curated By

  • BioGRID