BAIT

VCP

ALS14, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TERA, p97
valosin containing protein
GO Process (18)
GO Function (8)
GO Component (13)
Homo sapiens
PREY

CNIH4

CNIH-4, HSPC163
cornichon family AMPA receptor auxiliary protein 4
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

AP-SWATH Reveals Direct Involvement of VCP/p97 in Integrated Stress Response Signaling Through Facilitating CReP/PPP1R15B Degradation.

Huelsmann J, Kravic B, Weith M, Gstaiger M, Aebersold R, Collins BC, Meyer H

The ubiquitin-directed AAA-ATPase VCP/p97 facilitates degradation of damaged or misfolded proteins in diverse cellular stress response pathways. Resolving the complexity of its interactions with partner and substrate proteins and understanding its links to stress signaling is therefore a major challenge. Here, we used affinity-purification SWATH mass spectrometry (AP-SWATH) to identify proteins that specifically interact with the substrate-trapping mutant, p97-E578Q. AP-SWATH ... [more]

Mol. Cell Proteomics Jul. 01, 2018; 17(7);1295-1307 [Pubmed: 29599191]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
VCP CNIH4
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2563023

Curated By

  • BioGRID