BAIT

PDZK1

CAP70, CLAMP, NHERF-3, NHERF3, PDZD1

PDZ domain containing 1

GO Process (7)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

carnitine transport [IDA]

cell proliferation [TAS]

drug transport [NAS]

positive regulation of ion transmembrane transport [IDA]

positive regulation of protein targeting to membrane [ISS]

regulation of anion transport [ISS]

transport [IDA]

Gene Ontology Molecular Function

PDZ domain binding [IPI]
protein binding [IPI]
transporter activity [TAS]

Gene Ontology Cellular Component

brush border membrane [ISS]

extracellular vesicular exosome [IDA]

plasma membrane [ISS]

CRISPR Database OMIM VEGA HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

UBE2I

C358B7.1, P18, UBC9, LA16c-358B7.1

ubiquitin-conjugating enzyme E2I

GO Process (8)

GO Function (9)

GO Component (6)

Gene Ontology Biological Process

cellular protein metabolic process [TAS]

cellular protein modification process [TAS]

negative regulation of transcription from RNA polymerase II promoter [IMP]

negative regulation of transcription, DNA-templated [IDA]

post-translational protein modification [TAS]

protein sumoylation [IDA, TAS]

protein ubiquitination [IBA]

ubiquitin-dependent protein catabolic process [TAS]

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]
SUMO transferase activity [IDA]
enzyme binding [IPI]
poly(A) RNA binding [IDA]
protein binding [IPI]
transcription factor binding [IPI]
ubiquitin protein ligase activity [IBA]
ubiquitin protein ligase binding [IBA]
ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleoplasm [TAS]

synaptonemal complex [TAS]

transferase complex [IDA]

CRISPR Database VEGA OMIM HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

PDZK1: I. a major scaffolder in brush borders of proximal tubular cells.

Gisler SM, Pribanic S, Bacic D, Forrer P, Gantenbein A, Sabourin LA, Tsuji A, Zhao ZS, Manser E, Biber J, Murer H

BACKGROUND: In proximal tubular cells, PDZK1 (NaPi-Cap1) has been implicated in apical expression of the Na+-dependent phosphate cotransporter (NaPi-IIa) via interaction with its C-terminus. PDZK1 represents a multidomain protein consisting of four PDZ domains and thus is believed to have a broader specificity besides NaPi-IIa. METHODS: We subjected single PDZ domains derived from PDZK1 either to yeast two-hybrid screens or ... [more]

Kidney Int. Nov. 01, 2003; 64(5);1733-45 [Pubmed: 14531806]

Throughput

Low Throughput

Curated By

BioGRID