Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.


Isolation and characterization of a novel ligand-dependent thyroid hormone receptor-coactivating protein.

Monden T, Wondisford FE, Hollenberg AN

The thyroid hormone receptor (TR) regulates the expression of target genes upon binding to triiodothyronine (T3) response elements. In the presence of T3, the TR recruits coactivating proteins that both modulate and integrate the ligand response. We report here the cloning of a novel protein using the TR ligand-binding domain as bait in the yeast two-hybrid system. Analysis of a ... [more]

J. Biol. Chem. Nov. 21, 1997; 272(47);29834-41 [Pubmed: 9368056]


  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.


Curated By

  • BioGRID