Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Hicks JA, Li L, Matsui M, Chu Y, Volkov O, Johnson KC, Corey DR

In the cytoplasm, small RNAs can control mammalian translation by regulating the stability of mRNA. In the nucleus, small RNAs can also control transcription and splicing. The mechanisms for RNA-mediated nuclear regulation are not understood and remain controversial, hindering the effective application of nuclear RNAi and investigation of its natural regulatory roles. Here, we reveal that the human GW182 paralogs ... [more]

Cell Rep Dec. 15, 2016; 20(7);1543-1552 [Pubmed: 28813667]

Throughput

  • High Throughput

Additional Notes

  • samples treated with DNase

Curated By

  • BioGRID