An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

N-CoR mediates DNA methylation-dependent repression through a methyl CpG binding protein Kaiso.

Yoon HG, Chan DW, Reynolds AB, Qin J, Wong J

The identification and characterization of molecular mechanisms utilized by cells to mediate transcriptional repression at methylated loci are fundamental to understanding the biological consequences of DNA methylation. Here we demonstrate that Kaiso, a methyl CpG binding protein belonging to the BTB/POZ family of transcription factors, is a component of the human N-CoR complex. In vitro, the Kaiso/N-CoR complex binds specific ... [more]

Mol. Cell Sep. 01, 2003; 12(3);723-34 [Pubmed: 14527417]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

NCOR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IPI]histone deacetylase binding [IPI]nuclear hormone receptor binding [IPI]protein binding [IPI]transcription corepressor activity [IDA, IMP]transcription regulatory region DNA binding [ISS]

Gene Ontology Cellular Component

HSPA4