Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Genetic interaction mapping in mammalian cells using CRISPR interference.

Du D, Roguev A, Gordon DE, Chen M, Chen SH, Shales M, Shen JP, Ideker T, Mali P, Qi LS, Krogan NJ

We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and ... [more]

Nat. Methods Jun. 01, 2017; 14(6);577-580 [Pubmed: 28481362]

Quantitative Score

-1.874827 [Confidence Score]

Throughput

High Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507)

Additional Notes

CRISPR GI screen
Cell Line: HEK293 (EFO:0001182)
Experimental Setup: Timecourse
GIST: A-phenotypic negative genetic interaction
Library: Targeted 107 gene chromatin regulation CRISPRi library
Significance Threshold: S score < -1

Curated By

BioGRID

Interaction Summary

SRCAP

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone acetyltransferase activity [TAS]protein binding [IPI]transcription coactivator activity [TAS]

Gene Ontology Cellular Component

THOC2