BAIT
CTR9
SH2BP1, TSBP, p150, p150TSP
CTR9, Paf1/RNA polymerase II complex component
GO Process (14)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
- JAK-STAT cascade [ISS]
- cellular response to lipopolysaccharide [ISS]
- endodermal cell fate commitment [ISS]
- histone H2B ubiquitination [IDA]
- histone H3-K4 trimethylation [IMP]
- histone monoubiquitination [IDA]
- interleukin-6-mediated signaling pathway [ISS]
- negative regulation of myeloid cell differentiation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of histone H3-K4 methylation [IMP]
- positive regulation of histone H3-K79 methylation [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- stem cell maintenance [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
MED15
ARC105, CAG7A, CTG7A, PCQAP, TIG-1, TIG1, TNRC7
mediator complex subunit 15
GO Process (2)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Genetic interaction mapping in mammalian cells using CRISPR interference.
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and ... [more]
Nat. Methods Jun. 01, 2017; 14(6);577-580 [Pubmed: 28481362]
Quantitative Score
- -1.224803 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line: HEK293 (EFO:0001182)
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Targeted 107 gene chromatin regulation CRISPRi library
- Significance Threshold: S score < -1
Curated By
- BioGRID