BAIT
PHC1
EDR1, HPH1, MCPH11, RAE28
polyhomeotic homolog 1 (Drosophila)
GO Process (1)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
SRCAP
DOMO1, EAF1, FLHS, SWR1
Snf2-related CREBBP activator protein
GO Process (2)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Genetic interaction mapping in mammalian cells using CRISPR interference.
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and ... [more]
Nat. Methods Jun. 01, 2017; 14(6);577-580 [Pubmed: 28481362]
Quantitative Score
- -1.094915 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line: HEK293 (EFO:0001182)
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Targeted 107 gene chromatin regulation CRISPRi library
- Significance Threshold: S score < -1
Curated By
- BioGRID