BAIT
ELP3
KAT9
elongator acetyltransferase complex subunit 3
GO Process (9)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
- central nervous system development [ISS]
- chromatin organization [TAS]
- histone H3 acetylation [IDA]
- histone H4 acetylation [IDA]
- neuron migration [ISS]
- positive regulation of cell migration [ISS]
- regulation of protein kinase activity [IDA]
- regulation of transcription from RNA polymerase II promoter [IDA, TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
EPC1
Epl1
enhancer of polycomb homolog 1 (Drosophila)
GO Process (9)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
- chromatin organization [TAS]
- histone H2A acetylation [IDA]
- histone H4 acetylation [IDA]
- negative regulation of gene expression, epigenetic [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [TAS]
- transcription, DNA-templated [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Genetic interaction mapping in mammalian cells using CRISPR interference.
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and ... [more]
Nat. Methods Jun. 01, 2017; 14(6);577-580 [Pubmed: 28481362]
Quantitative Score
- -1.066213 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line: HEK293 (EFO:0001182)
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Targeted 107 gene chromatin regulation CRISPRi library
- Significance Threshold: S score < -1
Curated By
- BioGRID