BAIT
APEX1
APE, APE1, APEN, APEX, APX, HAP1, REF1
APEX nuclease (multifunctional DNA repair enzyme) 1
GO Process (9)
GO Function (19)
GO Component (10)
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IDA, TAS]
- DNA catabolic process, exonucleolytic [IBA]
- DNA demethylation [IDA]
- DNA repair [IDA, TAS]
- base-excision repair [IBA, TAS]
- negative regulation of nucleic acid-templated transcription [TAS]
- oxidation-reduction process [IDA]
- positive regulation of DNA repair [IDA]
- regulation of mRNA stability [IMP]
Gene Ontology Molecular Function- 3'-5' exonuclease activity [IDA, TAS]
- DNA binding [IDA]
- DNA-(apurinic or apyrimidinic site) lyase activity [IDA, TAS]
- RNA-DNA hybrid ribonuclease activity [TAS]
- chromatin DNA binding [IDA]
- damaged DNA binding [IDA]
- double-stranded DNA 3'-5' exodeoxyribonuclease activity [IBA]
- endodeoxyribonuclease activity [TAS]
- endonuclease activity [IDA]
- metal ion binding [IDA]
- oxidoreductase activity [IDA]
- phosphodiesterase I activity [TAS]
- phosphoric diester hydrolase activity [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [TAS]
- uracil DNA N-glycosylase activity [TAS]
- 3'-5' exonuclease activity [IDA, TAS]
- DNA binding [IDA]
- DNA-(apurinic or apyrimidinic site) lyase activity [IDA, TAS]
- RNA-DNA hybrid ribonuclease activity [TAS]
- chromatin DNA binding [IDA]
- damaged DNA binding [IDA]
- double-stranded DNA 3'-5' exodeoxyribonuclease activity [IBA]
- endodeoxyribonuclease activity [TAS]
- endonuclease activity [IDA]
- metal ion binding [IDA]
- oxidoreductase activity [IDA]
- phosphodiesterase I activity [TAS]
- phosphoric diester hydrolase activity [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [TAS]
- uracil DNA N-glycosylase activity [TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
ATM
AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1
ATM serine/threonine kinase
GO Process (23)
GO Function (7)
GO Component (2)
Gene Ontology Biological Process
- DNA damage induced protein phosphorylation [IDA]
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]
- DNA repair [TAS]
- cell cycle arrest [IMP]
- cellular response to DNA damage stimulus [IMP]
- cellular response to gamma radiation [IDA]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [TAS]
- histone mRNA catabolic process [IDA]
- mitotic spindle assembly checkpoint [IMP]
- negative regulation of B cell proliferation [IMP]
- peptidyl-serine phosphorylation [IDA]
- phosphatidylinositol-3-phosphate biosynthetic process [IMP]
- positive regulation of DNA damage response, signal transduction by p53 class mediator [IMP]
- positive regulation of apoptotic process [IMP]
- pre-B cell allelic exclusion [ISS]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- reciprocal meiotic recombination [TAS]
- replicative senescence [IMP]
- response to ionizing radiation [IDA]
- signal transduction [TAS]
- signal transduction involved in mitotic G2 DNA damage checkpoint [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -4.714 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID