BAIT
LIG1
ligase I, DNA, ATP-dependent
GO Process (16)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- DNA metabolic process [TAS]
- DNA repair [TAS]
- DNA strand elongation involved in DNA replication [TAS]
- V(D)J recombination [IDA]
- anatomical structure morphogenesis [TAS]
- base-excision repair [TAS]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [TAS]
- lagging strand elongation [IBA]
- mitotic cell cycle [TAS]
- nucleotide-excision repair [IBA, TAS]
- nucleotide-excision repair, DNA gap filling [TAS]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NAMPT
1110035O14Rik, PBEF, PBEF1, VF, VISFATIN
nicotinamide phosphoribosyltransferase
GO Process (13)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- NAD metabolic process [TAS]
- adipose tissue development [IDA]
- cell-cell signaling [TAS]
- circadian regulation of gene expression [ISS]
- insulin receptor signaling pathway [IDA]
- nicotinamide metabolic process [TAS]
- positive regulation of cell proliferation [TAS]
- positive regulation of nitric-oxide synthase biosynthetic process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -4.511 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID