BAIT
GCK
FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2
glucokinase (hexokinase 4)
GO Process (17)
GO Function (6)
GO Component (2)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cellular glucose homeostasis [IBA]
- cellular response to insulin stimulus [ISS]
- cellular response to leptin stimulus [ISS]
- detection of glucose [IMP]
- endocrine pancreas development [TAS]
- glucose homeostasis [IMP]
- glucose transport [TAS]
- hexose transport [TAS]
- negative regulation of gluconeogenesis [IMP]
- positive regulation of glycogen biosynthetic process [IMP]
- positive regulation of insulin secretion [IMP]
- regulation of glucose transport [TAS]
- regulation of glycolytic process [NAS]
- regulation of insulin secretion [IMP]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [TAS]
- nucleoplasm [TAS]
Homo sapiens
PREY
ITGB7
integrin, beta 7
GO Process (10)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- cell adhesion [TAS]
- cell-matrix adhesion [IMP]
- cell-matrix adhesion involved in ameboidal cell migration [IMP]
- extracellular matrix organization [TAS]
- heterotypic cell-cell adhesion [IMP]
- integrin-mediated signaling pathway [IMP]
- leukocyte tethering or rolling [IMP]
- receptor clustering [IMP]
- regulation of immune response [TAS]
- substrate adhesion-dependent cell spreading [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -3.455 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID