BAIT
PIM2
Pim-2 proto-oncogene, serine/threonine kinase
GO Process (13)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IDA]
- cell proliferation [TAS]
- male meiosis [TAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of cell proliferation [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISS]
- positive regulation of autophagy [ISS]
- positive regulation of transcription, DNA-templated [ISS]
- protein autophosphorylation [IBA]
- protein phosphorylation [IDA, ISS]
- protein stabilization [IMP, ISS]
- regulation of mitotic cell cycle [IBA]
- response to virus [IEP]
Gene Ontology Molecular Function
Homo sapiens
PREY
TOP1
TOPI, RP3-511B24.1
topoisomerase (DNA) I
GO Process (9)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -2.754 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID