BAIT
NOS2
HEP-NOS, INOS, NOS, NOS2A
nitric oxide synthase 2, inducible
GO Process (21)
GO Function (11)
GO Component (5)
Gene Ontology Biological Process
- arginine catabolic process [IDA]
- blood coagulation [TAS]
- defense response to Gram-negative bacterium [NAS]
- defense response to bacterium [IMP, ISS]
- inflammatory response [IBA]
- innate immune response in mucosa [NAS]
- interaction with host [TAS]
- negative regulation of blood pressure [IBA]
- negative regulation of gene expression [IGI]
- nitric oxide biosynthetic process [IDA]
- nitric oxide mediated signal transduction [IBA]
- peptidyl-cysteine S-nitrosylation [ISS]
- phagosome maturation [TAS]
- positive regulation of guanylate cyclase activity [IBA]
- positive regulation of killing of cells of other organism [IMP]
- positive regulation of leukocyte mediated cytotoxicity [TAS]
- positive regulation of vasodilation [IBA]
- regulation of cellular respiration [TAS]
- regulation of insulin secretion [IMP]
- response to bacterium [NAS]
- superoxide metabolic process [ISS]
Gene Ontology Molecular Function- FMN binding [ISS]
- NADP binding [TAS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- nitric-oxide synthase activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [ISS]
- receptor binding [IPI]
- tetrahydrobiopterin binding [ISS]
- FMN binding [ISS]
- NADP binding [TAS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- nitric-oxide synthase activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [ISS]
- receptor binding [IPI]
- tetrahydrobiopterin binding [ISS]
Homo sapiens
PREY
PIK3CG
PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma
GO Process (25)
GO Function (7)
GO Component (6)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- T cell activation [TAS]
- T cell chemotaxis [TAS]
- T cell proliferation [TAS]
- adaptive immune response [TAS]
- blood coagulation [TAS]
- cytokine production [TAS]
- dendritic cell chemotaxis [TAS]
- inflammatory response [TAS]
- innate immune response [TAS]
- mast cell degranulation [TAS]
- natural killer cell chemotaxis [TAS]
- negative regulation of cardiac muscle contraction [TAS]
- neutrophil chemotaxis [TAS]
- neutrophil extravasation [TAS]
- phosphatidylinositol 3-kinase signaling [IDA, TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phospholipid metabolic process [TAS]
- platelet activation [TAS]
- platelet aggregation [TAS]
- positive regulation of MAP kinase activity [IDA]
- positive regulation of protein kinase B signaling [IDA]
- regulation of cell adhesion mediated by integrin [TAS]
- respiratory burst involved in defense response [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -2.714 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID