BAIT

MAPK8IP1

IB1, JIP-1, JIP1, PRKM8IP
mitogen-activated protein kinase 8 interacting protein 1
GO Process (2)
GO Function (4)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.

Han K, Jeng EE, Hess GT, Morgens DW, Li A, Bassik MC

Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]

Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]

Quantitative Score

  • 3.019 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507)

Additional Notes

  • CRISPR GI screen
  • Cell Line:K562 (EFO:0002067)
  • Experimental Setup:Timecourse
  • GIST: A-phenotypic positive genetic interaction
  • Library:Drug Target-CDKO CRISPRn library
  • Significance Threshold: q-value<0.05

Curated By

  • BioGRID