FUT8
Gene Ontology Biological Process
- GDP-L-fucose metabolic process [IDA]
- L-fucose catabolic process [NAS]
- N-glycan fucosylation [IDA, TAS]
- N-glycan processing [TAS]
- cellular protein metabolic process [TAS]
- in utero embryonic development [NAS]
- oligosaccharide biosynthetic process [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation [TAS]
- protein N-linked glycosylation via asparagine [IDA, TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MAN2A1
Gene Ontology Biological Process
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Quantitative Score
- -16.385 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Toxin Exposure: Ricin at LD50 (first two pulses: 0.25 ng/ml ricin, third pulse: 0.3 ng/ml, fourth pulse: 0.35 ng/ml)
- GIST: A-phenotypic negative genetic interaction
- Library:Ricin-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FUT8 MAN2A1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9915 | BioGRID | 2240416 | |
| FUT8 MAN2A1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9851 | BioGRID | 3116686 |
Curated By
- BioGRID