Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.

Han K, Jeng EE, Hess GT, Morgens DW, Li A, Bassik MC

Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]

Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]

Quantitative Score

  • -16.385 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507)

Additional Notes

  • CRISPR GI screen
  • Cell Line:K562 (EFO:0002067)
  • Experimental Setup:Toxin Exposure: Ricin at LD50 (first two pulses: 0.25 ng/ml ricin, third pulse: 0.3 ng/ml, fourth pulse: 0.35 ng/ml)
  • GIST: A-phenotypic negative genetic interaction
  • Library:Ricin-CDKO CRISPRn library
  • Significance Threshold: q-value<0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FUT8 MAN2A1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9915BioGRID
2240416

Curated By

  • BioGRID