BAIT
CAND1
TIP120, TIP120A
cullin-associated and neddylation-dissociated 1
GO Process (4)
GO Function (1)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GCNT2
CCAT, CTRCT13, GCNT2C, GCNT5, IGNT, II, NACGT1, NAGCT1, ULG3, bA360O19.2, bA421M1.1, RP11-421M1.1
glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
GO Process (13)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- glycosaminoglycan biosynthetic process [TAS]
- maintenance of lens transparency [IMP]
- multicellular organismal development [TAS]
- negative regulation of cell-substrate adhesion [IMP, ISS]
- positive regulation of ERK1 and ERK2 cascade [IMP, ISS]
- positive regulation of cell migration [IMP, ISS]
- positive regulation of cell proliferation [ISS]
- positive regulation of epithelial to mesenchymal transition [IMP, ISS]
- positive regulation of heterotypic cell-cell adhesion [IMP, ISS]
- positive regulation of protein kinase B signaling [IMP, ISS]
- posttranscriptional regulation of gene expression [IMP, ISS]
- protein glycosylation [IMP]
- transforming growth factor beta receptor signaling pathway [IMP, ISS]
Gene Ontology Molecular Function
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -10.402 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Toxin Exposure: Ricin at LD50 (first two pulses: 0.25 ng/ml ricin, third pulse: 0.3 ng/ml, fourth pulse: 0.35 ng/ml)
- GIST: A-phenotypic negative genetic interaction
- Library:Ricin-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID