BAIT
B4GALT1
B4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
GO Process (14)
GO Function (9)
GO Component (12)
Gene Ontology Biological Process
- Notch signaling pathway [TAS]
- binding of sperm to zona pellucida [TAS]
- carbohydrate metabolic process [TAS]
- cellular protein metabolic process [TAS]
- glycosaminoglycan metabolic process [TAS]
- keratan sulfate biosynthetic process [TAS]
- keratan sulfate metabolic process [TAS]
- multicellular organism reproduction [TAS]
- oligosaccharide biosynthetic process [IDA, NAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation [IDA]
- protein N-linked glycosylation via asparagine [TAS]
- single fertilization [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- N-acetyllactosamine synthase activity [IDA]
- UDP-galactosyltransferase activity [IDA]
- alpha-tubulin binding [IDA]
- beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity [IDA]
- beta-tubulin binding [IPI]
- galactosyltransferase activity [NAS]
- lactose synthase activity [IDA]
- manganese ion binding [IDA]
- protein homodimerization activity [IDA]
- N-acetyllactosamine synthase activity [IDA]
- UDP-galactosyltransferase activity [IDA]
- alpha-tubulin binding [IDA]
- beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity [IDA]
- beta-tubulin binding [IPI]
- galactosyltransferase activity [NAS]
- lactose synthase activity [IDA]
- manganese ion binding [IDA]
- protein homodimerization activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
TRAPPC13
C5orf44
trafficking protein particle complex 13
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -5.678 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Toxin Exposure: Ricin at LD50 (first two pulses: 0.25 ng/ml ricin, third pulse: 0.3 ng/ml, fourth pulse: 0.35 ng/ml)
- GIST: A-phenotypic negative genetic interaction
- Library:Ricin-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID