BAIT
B4GALT1
B4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
GO Process (14)
GO Function (9)
GO Component (12)
Gene Ontology Biological Process
- Notch signaling pathway [TAS]
- binding of sperm to zona pellucida [TAS]
- carbohydrate metabolic process [TAS]
- cellular protein metabolic process [TAS]
- glycosaminoglycan metabolic process [TAS]
- keratan sulfate biosynthetic process [TAS]
- keratan sulfate metabolic process [TAS]
- multicellular organism reproduction [TAS]
- oligosaccharide biosynthetic process [IDA, NAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation [IDA]
- protein N-linked glycosylation via asparagine [TAS]
- single fertilization [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- N-acetyllactosamine synthase activity [IDA]
- UDP-galactosyltransferase activity [IDA]
- alpha-tubulin binding [IDA]
- beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity [IDA]
- beta-tubulin binding [IPI]
- galactosyltransferase activity [NAS]
- lactose synthase activity [IDA]
- manganese ion binding [IDA]
- protein homodimerization activity [IDA]
- N-acetyllactosamine synthase activity [IDA]
- UDP-galactosyltransferase activity [IDA]
- alpha-tubulin binding [IDA]
- beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity [IDA]
- beta-tubulin binding [IPI]
- galactosyltransferase activity [NAS]
- lactose synthase activity [IDA]
- manganese ion binding [IDA]
- protein homodimerization activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
DEPDC5
DEP.5, FFEVF, LL22NC03-113A11.1
DEP domain containing 5
GO Process (0)
GO Function (0)
GO Component (3)
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -4.684 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Toxin Exposure: Ricin at LD50 (first two pulses: 0.25 ng/ml ricin, third pulse: 0.3 ng/ml, fourth pulse: 0.35 ng/ml)
- GIST: A-phenotypic negative genetic interaction
- Library:Ricin-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID