BAIT
DEPDC5
DEP.5, FFEVF, LL22NC03-113A11.1
DEP domain containing 5
GO Process (0)
GO Function (0)
GO Component (3)
Gene Ontology Cellular Component
Homo sapiens
PREY
GCNT2
CCAT, CTRCT13, GCNT2C, GCNT5, IGNT, II, NACGT1, NAGCT1, ULG3, bA360O19.2, bA421M1.1, RP11-421M1.1
glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
GO Process (13)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- glycosaminoglycan biosynthetic process [TAS]
- maintenance of lens transparency [IMP]
- multicellular organismal development [TAS]
- negative regulation of cell-substrate adhesion [IMP, ISS]
- positive regulation of ERK1 and ERK2 cascade [IMP, ISS]
- positive regulation of cell migration [IMP, ISS]
- positive regulation of cell proliferation [ISS]
- positive regulation of epithelial to mesenchymal transition [IMP, ISS]
- positive regulation of heterotypic cell-cell adhesion [IMP, ISS]
- positive regulation of protein kinase B signaling [IMP, ISS]
- posttranscriptional regulation of gene expression [IMP, ISS]
- protein glycosylation [IMP]
- transforming growth factor beta receptor signaling pathway [IMP, ISS]
Gene Ontology Molecular Function
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -3.684 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Toxin Exposure: Ricin at LD50 (first two pulses: 0.25 ng/ml ricin, third pulse: 0.3 ng/ml, fourth pulse: 0.35 ng/ml)
- GIST: A-phenotypic negative genetic interaction
- Library:Ricin-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID