Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.

Han K, Jeng EE, Hess GT, Morgens DW, Li A, Bassik MC

Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]

Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]

Quantitative Score

-1.493 [Confidence Score]

Throughput

High Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507)

Additional Notes

CRISPR GI screen
Cell Line:K562 (EFO:0002067)
Experimental Setup:Toxin Exposure: Ricin at LD50 (first two pulses: 0.25 ng/ml ricin, third pulse: 0.3 ng/ml, fourth pulse: 0.35 ng/ml)
GIST: A-phenotypic negative genetic interaction
Library:Ricin-CDKO CRISPRn library
Significance Threshold: q-value<0.05

Curated By

BioGRID

Interaction Summary

CBL

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3 domain binding [IPI]ephrin receptor binding [IPI]protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

KHSRP