An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Adeno-associated virus Rep proteins antagonize phosphatase PP1 to counteract KAP1 repression of the latent viral genome.

Smith-Moore S, Neil SJD, Fraefel C, Linden RM, Bollen M, Rowe HM, Henckaerts E

Adeno-associated virus (AAV) is a small human Dependovirus whose low immunogenicity and capacity for long-term persistence have led to its widespread use as vector for gene therapy. Despite great recent successes in AAV-based gene therapy, further improvements in vector technology may be hindered by an inadequate understanding of various aspects of basic AAV biology. AAV is unique in that its ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 10, 2017; 115(15);E3529-E3538 [Pubmed: 29581310]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PPP1R8 SETDB1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Stelzl U (2005)	High	1	BioGRID	-

Curated By

BioGRID

Interaction Summary

PPP1R8

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein serine/threonine phosphatase inhibitor activity [TAS]ribonuclease E activity [TAS]

Gene Ontology Cellular Component

SETDB1