BAIT

TFAP2A

AP-2, AP-2alpha, AP2TF, BOFS, TFAP2, RP1-290I10.1
transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
GO Process (28)
GO Function (15)
GO Component (6)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Tyrosine phosphorylation of the beta2 subunit of clathrin adaptor complex AP-2 reveals the role of a di-leucine motif in the epidermal growth factor receptor trafficking.

Huang F, Jiang X, Sorkin A

Tyrosine phosphorylation of the beta2 subunit of clathrin adaptor complex AP-2 was detected in three types of cells treated with epidermal growth factor (EGF). The tyrosine phosphorylation was observed during recruitment of EGF receptors into coated pits at 4 degrees C and reached maximum at 37 degrees C at post-recruitment stages of endocytosis. An inhibitor of EGF receptor kinase completely ... [more]

J. Biol. Chem. Oct. 31, 2003; 278(44);43411-7 [Pubmed: 12900408]

Throughput

  • Low Throughput

Curated By

  • BioGRID