An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mitochondrial AKAP121 binds and targets protein tyrosine phosphatase D1, a novel positive regulator of src signaling.

Cardone L, Carlucci A, Affaitati A, Livigni A, DeCristofaro T, Garbi C, Varrone S, Ullrich A, Gottesman ME, Avvedimento EV, Feliciello A

A-kinase anchor protein 121 (AKAP121) and its spliced isoform AKAP84 anchor protein kinase A (PKA) to the outer membrane of mitochondria, focusing and enhancing cyclic AMP signal transduction to the organelle. We find that AKAP121/84 also binds PTPD1, a src-associated protein tyrosine phosphatase. A signaling complex containing AKAP121, PKA, PTPD1, and src is assembled in vivo. PTPD1 activates src tyrosine ... [more]

Mol. Cell. Biol. Jun. 01, 2004; 24(11);4613-26 [Pubmed: 15143158]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PTPN21 AKAP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Cardone L (2004)	Low	-	BioGRID	-
PTPN21 AKAP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Cardone L (2004)	Low	-	BioGRID	-
AKAP1 PTPN21	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Cardone L (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AKAP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

beta-tubulin binding [IDA]microtubule binding [IDA]poly(A) RNA binding [ISO]protein complex scaffold [IDA]protein domain specific binding [IMP]protein kinase A regulatory subunit binding [IDA]protein kinase binding [IDA]protein phosphatase 2B binding [IDA]protein phosphatase binding [IDA]

Gene Ontology Cellular Component

PTPN21