BAIT
SPF1
COD1, PER9, PIO1, ion-transporting P-type ATPase SPF1, L000003454, YEL031W
P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
GO Process (4)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
OSH3
oxysterol-binding protein related protein OSH3, YHR073W
Member of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
GO Process (9)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- ER to Golgi ceramide transport [IMP]
- endocytosis [IGI]
- exocytosis [IGI]
- invasive growth in response to glucose limitation [IGI]
- karyogamy involved in conjugation with cellular fusion [IGI]
- maintenance of cell polarity [IGI]
- positive regulation of phosphatase activity [IDA]
- pseudohyphal growth [IMP]
- sterol transport [IDA, IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
The P5A ATPase Spf1p is stimulated by phosphatidylinositol 4-phosphate and influences cellular sterol homeostasis.
P5A ATPases are expressed in the endoplasmic reticulum (ER) of all eukaryotic cells, and their disruption results in severe ER stress. However, the function of these ubiquitous membrane proteins, which belong to the P-type ATPase superfamily, is unknown. We purified a functional tagged version of the Saccharomyces cerevisiae P5A ATPase Spf1p and observed that the ATP hydrolytic activity of the ... [more]
Mol. Biol. Cell Feb. 20, 2019; ();mbcE18060365 [Pubmed: 30785834]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID