BAIT

YJL049W

Putative protein of unknown function; YJL049W is a non-essential gene

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

VPS20

CHM6, VPL10, VPT20, ESCRT-III subunit protein VPS20, YMR077C

Myristoylated subunit of the ESCRT-III complex; the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes

GO Process (3)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

intralumenal vesicle formation [IGI, IMP]

late endosome to vacuole transport [IMP]

ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IC]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

ESCRT III complex [IDA]

cytoplasm [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

An ESCRT-LEM protein surveillance system is poised to directly monitor the nuclear envelope and nuclear transport system.

Thaller DJ, Allegretti M, Borah S, Ronchi P, Beck M, Lusk CP

The integrity of the nuclear membranes coupled to the selective barrier of nuclear pore complexes (NPCs) are essential for the segregation of nucleoplasm and cytoplasm. Mechanical membrane disruption or perturbation to NPC assembly triggers an ESCRT-dependent surveillance system that seals nuclear pores: how these pores are sensed and sealed is ill defined. Using a budding yeast model, we show that ... [more]

Elife Apr. 03, 2019; 8(); [Pubmed: 30942170]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary