BAIT

XPO1

CRM1, emb, exp1
exportin 1
Human Papilloma Virus (HPV) Project
Homo sapiens
PREY

RNF146

dJ351K20.1, RP3-351K20.1
ring finger protein 146
UPS Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

XPO1-mediated nuclear export of RNF146 protects from angiotensin II-induced endothelial cellular injury.

Sheng Z, Xu Y, Wang S, Yuan Y, Huang T, Lu P

Endothelial cells death induced by angiotensin II (Ang II) plays a role in vascular injury. RNF146 is identified as a E3 ubiquitin ligase, which promotes cell survival under many types of stresses. However, the role of RNF146 in endothelial cellular injury is unknown. In human umbilical vein endothelial cells (HUVECs), Ang II treatment led to cell death by oxidative stress ... [more]

Biochem. Biophys. Res. Commun. Dec. 10, 2017; 503(3);1544-1549 [Pubmed: 30029878]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
XPO1 RNF146
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
1439217

Curated By

  • BioGRID