BAIT

WWTR1

2310058J06Rik, 2610021I22Rik, C78399, Taz
WW domain containing transcription regulator 1
GO Process (24)
GO Function (4)
GO Component (4)
Mus musculus
PREY

YWHAQ

2700028P07Rik, AA409740, AU021156, R74690
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TAZ, a transcriptional modulator of mesenchymal stem cell differentiation.

Hong JH, Hwang ES, McManus MT, Amsterdam A, Tian Y, Kalmukova R, Mueller E, Benjamin T, Spiegelman BM, Sharp PA, Hopkins N, Yaffe MB

Mesenchymal stem cells (MSCs) are a pluripotent cell type that can differentiate into several distinct lineages. Two key transcription factors, Runx2 and peroxisome proliferator-activated receptor gamma (PPARgamma), drive MSCs to differentiate into either osteoblasts or adipocytes, respectively. How these two transcription factors are regulated in order to specify these alternate cell fates remains a pivotal question. Here we report that ... [more]

Science Aug. 12, 2005; 309(5737);1074-8 [Pubmed: 16099986]

Throughput

  • Low Throughput

Curated By

  • BioGRID