PREY

CRBN

MRT2, MRT2A, AD-006
cereblon
GO Process (2)
GO Function (1)
GO Component (4)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CRL4 antagonizes SCFFbxo7-mediated turnover of cereblon and BK channel to regulate learning and memory.

Song T, Liang S, Liu J, Zhang T, Yin Y, Geng C, Gao S, Feng Y, Xu H, Guo D, Roberts A, Gu Y, Cang Y

Intellectual disability (ID), one of the most common human developmental disorders, can be caused by genetic mutations in Cullin 4B (Cul4B) and cereblon (CRBN). CRBN is a substrate receptor for the Cul4A/B-DDB1 ubiquitin ligase (CRL4) and can target voltage- and calcium-activated BK channel for ER retention. Here we report that ID-associated CRL4CRBN mutations abolish the interaction of the BK channel ... [more]

PLoS Genet. Dec. 01, 2017; 14(1);e1007165 [Pubmed: 29370161]

Throughput

  • Low Throughput

Curated By

  • BioGRID