PREY

PCNA

ATLD2
proliferating cell nuclear antigen
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Human NTH1 physically interacts with p53 and proliferating cell nuclear antigen.

Oyama M, Wakasugi M, Hama T, Hashidume H, Iwakami Y, Imai R, Hoshino S, Morioka H, Ishigaki Y, Nikaido O, Matsunaga T

Thymine glycol (Tg) is one of predominant oxidative DNA lesions caused by ionizing radiation and other oxidative stresses. Human NTH1 is a bifunctional enzyme with DNA glycosylase and AP lyase activities and removes Tg as the first step of base excision repair (BER). We have searched for the factors interacting with NTH1 by using a pull-down assay and found that ... [more]

Biochem. Biophys. Res. Commun. Aug. 13, 2004; 321(1);183-91 [Pubmed: 15358233]

Throughput

  • Low Throughput

Curated By

  • BioGRID