BAIT

ARL8B

ARL10C, Gie1
ADP-ribosylation factor-like 8B
GO Process (2)
GO Function (5)
GO Component (5)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes.

Khatter D, Raina VB, Dwivedi D, Sindhwani A, Bahl S, Sharma M

The homotypic fusion and protein sorting (HOPS) complex is a multi-subunit complex conserved from yeast to mammals that regulates late endosome and lysosome fusion. However, little is known about how the HOPS complex is recruited to lysosomes in mammalian cells. Here, we report that the small GTPase Arl8b, but not Rab7 (also known as RAB7A), is essential for membrane localization ... [more]

J. Cell. Sci. May. 01, 2015; 128(9);1746-61 [Pubmed: 25908847]

Throughput

  • Low Throughput

Curated By

  • BioGRID