BAIT

ROM2

Rho family guanine nucleotide exchange factor ROM2, L000003182, YLR371W
GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

GTR1

Rag GTPase GTR1, L000000742, YML121W
Cytoplasmic GTPase; forms a heterodimer with Gtr2p to stimulate TORC1 in response to amino acids; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Multi-layered regulation of TORC1-body formation in budding yeast.

Sullivan A, Wallace RL, Wellington R, Luo X, Capaldi AP

The Target of Rapamycin Kinase Complex 1 (TORC1) regulates cell growth and metabolism in eukaryotes. In Saccharomyces cerevisiae, TORC1 activity is known to be controlled by the conserved GTPases, Gtr1/2, and movement into and out of an inactive agglomerate/body. However, it is unclear if/how these regulatory steps are coupled. Here we show that active Gtr1/2 is a potent inhibitor of ... [more]

Mol. Biol. Cell Nov. 28, 2018; ();mbcE18050297 [Pubmed: 30485160]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: protein/peptide accumulation (APO:0000149)

Additional Notes

  • Deletion of Gtr1 rescued the TORC1-body formation defects seen in the single mutants

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GTR1 ROM2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1634BioGRID
2160163

Curated By

  • BioGRID