PRKAB1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NPM1
Gene Ontology Biological Process
- DNA repair [ISO, ISS]
- cell aging [ISO, ISS]
- cell growth [ISO]
- cell volume homeostasis [ISO]
- centrosome cycle [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of cell proliferation [ISO, ISS]
- negative regulation of centrosome duplication [ISO]
- negative regulation of mRNA splicing, via spliceosome [ISO]
- negative regulation of protein kinase activity by regulation of protein phosphorylation [ISO, ISS]
- nucleocytoplasmic transport [ISO]
- nucleosome assembly [ISO]
- positive regulation of DNA metabolic process [IDA]
- positive regulation of DNA replication [IDA]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of catalytic activity [IDA]
- positive regulation of cell proliferation [ISO]
- positive regulation of cellular biosynthetic process [ISO]
- positive regulation of centrosome duplication [ISO]
- positive regulation of protein kinase activity [ISO]
- positive regulation of translation [ISO, ISS]
- protein destabilization [ISO]
- protein homooligomerization [IDA]
- protein localization [ISO]
- protein oligomerization [ISO]
- rRNA export from nucleus [ISO]
- regulation of DNA damage response, signal transduction by p53 class mediator [ISO]
- regulation of cell cycle [ISO]
- regulation of centriole replication [ISO, ISS]
- regulation of centrosome duplication [ISO]
- regulation of eIF2 alpha phosphorylation by dsRNA [ISO, ISS]
- regulation of endodeoxyribonuclease activity [ISO, ISS]
- regulation of endoribonuclease activity [ISO, ISS]
- regulation of neuron apoptotic process [IMP]
- ribosomal large subunit biogenesis [ISO]
- ribosomal large subunit export from nucleus [ISO]
- ribosomal small subunit biogenesis [ISO]
- ribosomal small subunit export from nucleus [ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- NF-kappaB binding [ISO, ISS]
- RNA binding [ISO]
- Tat protein binding [ISO]
- enzyme binding [IDA, IPI]
- histone binding [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [IPI]
- poly(A) RNA binding [ISO]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase binding [ISO]
- protein kinase inhibitor activity [ISO, ISS]
- rRNA binding [ISO]
- ribosomal large subunit binding [ISO]
- ribosomal small subunit binding [ISO]
- transcription coactivator activity [ISO]
- unfolded protein binding [ISO, ISS]
- DNA binding [IDA]
- NF-kappaB binding [ISO, ISS]
- RNA binding [ISO]
- Tat protein binding [ISO]
- enzyme binding [IDA, IPI]
- histone binding [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [IPI]
- poly(A) RNA binding [ISO]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase binding [ISO]
- protein kinase inhibitor activity [ISO, ISS]
- rRNA binding [ISO]
- ribosomal large subunit binding [ISO]
- ribosomal small subunit binding [ISO]
- transcription coactivator activity [ISO]
- unfolded protein binding [ISO, ISS]
Gene Ontology Cellular Component
- cell [ISO]
- centrosome [ISO, ISS]
- cytoplasm [ISO, ISS]
- cytosol [ISO]
- focal adhesion [ISO]
- granular component [ISO]
- intracellular [ISO]
- large ribosomal subunit [ISO]
- membrane [ISO]
- nuclear speck [ISO]
- nucleolus [IDA, ISO]
- nucleoplasm [ISO]
- nucleus [ISO, ISS]
- ribonucleoprotein complex [ISO]
- small ribosomal subunit [ISO]
- spindle pole centrosome [ISO]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Throughput
- High Throughput
Additional Notes
- Hit identified (by MS) from INS-1 lysates incubated with affinity purified PRKAB1
Curated By
- BioGRID