BAIT

PRKAB1

protein kinase, AMP-activated, beta 1 non-catalytic subunit
Rattus norvegicus
PREY

RPL11

ribosomal protein L11
GO Process (4)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.

Moon S, Han D, Kim Y, Jin J, Ho WK, Kim Y

The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]

Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]

Throughput

  • High Throughput

Additional Notes

  • Hit identified (by MS) from INS-1 lysates incubated with affinity purified PRKAB1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PRKAB1 RPL11
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID