BAIT
PRKAA1
AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- cellular response to ethanol [IEP]
- cellular response to glucose starvation [ISO, ISS]
- cellular response to hydrogen peroxide [IEP]
- cellular response to hypoxia [IEP]
- cellular response to nutrient levels [ISO, ISS]
- cellular response to organonitrogen compound [IEP]
- cold acclimation [IDA]
- fatty acid homeostasis [IDA]
- fatty acid oxidation [ISO]
- glucose homeostasis [ISO, ISS]
- glucose metabolic process [ISO]
- histone-serine phosphorylation [ISO]
- lipid biosynthetic process [ISO, ISS]
- negative regulation of TOR signaling [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of glucose import in response to insulin stimulus [IMP]
- negative regulation of lipid catabolic process [ISO, ISS]
- negative regulation of translation [NAS]
- positive regulation of autophagy [ISO, ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of fatty acid oxidation [NAS]
- positive regulation of gene expression [ISO]
- positive regulation of gluconeogenesis [NAS]
- positive regulation of glucose import [NAS]
- positive regulation of glycolytic process [IDA]
- protein heterooligomerization [IDA]
- protein phosphorylation [IDA, ISO, NAS]
- regulation of circadian rhythm [ISO, ISS]
- regulation of energy homeostasis [IDA]
- regulation of vesicle-mediated transport [IMP]
- response to UV [ISO]
- response to activity [IDA]
- response to caffeine [IDA]
- response to camptothecin [ISO]
- response to gamma radiation [ISO, ISS]
- response to hydrogen peroxide [ISO]
Gene Ontology Molecular Function- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
PPP2CA
Pp2a1
protein phosphatase 2, catalytic subunit, alpha isozyme
GO Process (26)
GO Function (16)
GO Component (9)
Gene Ontology Biological Process
- cardiac ventricle development [IEP]
- cellular response to calcium ion [IEP]
- cellular response to cytokine stimulus [IEP]
- cellular response to ethanol [IEP]
- cellular response to follicle-stimulating hormone stimulus [IEP]
- cellular response to glucose stimulus [IEP, IMP]
- cellular response to insulin stimulus [IEP]
- cellular response to steroid hormone stimulus [IEP]
- heart development [IEP]
- mesoderm development [ISO]
- negative regulation of calcium ion transmembrane transporter activity [IMP]
- negative regulation of epithelial to mesenchymal transition [ISO]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of transcription factor import into nucleus [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- positive regulation of protein dephosphorylation [IDA]
- positive regulation of protein phosphatase type 2A activity [IMP]
- positive regulation of protein serine/threonine kinase activity [ISO]
- protein dephosphorylation [IDA]
- protein heterotrimerization [IMP]
- regulation of protein autophosphorylation [IMP]
- regulation of protein catabolic process [IMP]
- regulation of protein phosphorylation [IMP]
- regulation of receptor activity [IMP]
Gene Ontology Molecular Function- GABA receptor binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI]
- protein C-terminus binding [IPI, ISO]
- protein antigen binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein dimerization activity [IMP]
- protein domain specific binding [IDA]
- protein heterodimerization activity [ISS]
- protein kinase B binding [IPI]
- protein kinase binding [IDA]
- protein phosphatase 2A binding [IDA, IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine phosphatase activity [IDA, IMP, TAS]
- tau protein binding [IPI]
- GABA receptor binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI]
- protein C-terminus binding [IPI, ISO]
- protein antigen binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein dimerization activity [IMP]
- protein domain specific binding [IDA]
- protein heterodimerization activity [ISS]
- protein kinase B binding [IPI]
- protein kinase binding [IDA]
- protein phosphatase 2A binding [IDA, IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine phosphatase activity [IDA, IMP, TAS]
- tau protein binding [IPI]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID