BAIT
PRKAA1
AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- cellular response to ethanol [IEP]
- cellular response to glucose starvation [ISO, ISS]
- cellular response to hydrogen peroxide [IEP]
- cellular response to hypoxia [IEP]
- cellular response to nutrient levels [ISO, ISS]
- cellular response to organonitrogen compound [IEP]
- cold acclimation [IDA]
- fatty acid homeostasis [IDA]
- fatty acid oxidation [ISO]
- glucose homeostasis [ISO, ISS]
- glucose metabolic process [ISO]
- histone-serine phosphorylation [ISO]
- lipid biosynthetic process [ISO, ISS]
- negative regulation of TOR signaling [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of glucose import in response to insulin stimulus [IMP]
- negative regulation of lipid catabolic process [ISO, ISS]
- negative regulation of translation [NAS]
- positive regulation of autophagy [ISO, ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of fatty acid oxidation [NAS]
- positive regulation of gene expression [ISO]
- positive regulation of gluconeogenesis [NAS]
- positive regulation of glucose import [NAS]
- positive regulation of glycolytic process [IDA]
- protein heterooligomerization [IDA]
- protein phosphorylation [IDA, ISO, NAS]
- regulation of circadian rhythm [ISO, ISS]
- regulation of energy homeostasis [IDA]
- regulation of vesicle-mediated transport [IMP]
- response to UV [ISO]
- response to activity [IDA]
- response to caffeine [IDA]
- response to camptothecin [ISO]
- response to gamma radiation [ISO, ISS]
- response to hydrogen peroxide [ISO]
Gene Ontology Molecular Function- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
MAP1B
Mtap1b
microtubule-associated protein 1B
GO Process (30)
GO Function (5)
GO Component (16)
Gene Ontology Biological Process
- axon extension [ISO]
- axonogenesis [ISO]
- cellular process [ISO]
- cellular response to growth factor stimulus [IEP]
- cellular response to peptide hormone stimulus [IEP]
- dendrite development [ISO]
- developmental maturation [IEP]
- establishment of monopolar cell polarity [ISO]
- induction of synaptic plasticity by chemical substance [IEP]
- microtubule bundle formation [IDA, ISO]
- mitochondrion transport along microtubule [ISO]
- negative regulation of intracellular transport [ISO]
- negative regulation of microtubule depolymerization [IDA]
- nervous system development [IEP]
- neuron development [IEP]
- peripheral nervous system axon regeneration [IEP]
- positive regulation of axon extension [IMP, ISO]
- positive regulation of microtubule polymerization [IDA]
- positive regulation of neuron differentiation [IMP]
- response to axon injury [IEP]
- response to carbohydrate [IEP]
- response to drug [IEP]
- response to estradiol [IEP]
- response to inorganic substance [IEP]
- response to insecticide [IEP]
- response to mechanical stimulus [IEP]
- response to nutrient levels [IEP]
- response to vitamin A [IEP]
- response to wounding [IEP]
- synapse assembly [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- axon [IDA]
- cell [ISO]
- cytoplasm [ISO]
- cytoskeleton [IDA]
- cytosol [ISO]
- dendrite [IDA]
- growth cone [IDA]
- intracellular [ISO]
- microtubule associated complex [IDA]
- neuronal cell body [IDA]
- neuronal postsynaptic density [ISO]
- perikaryon [IDA]
- perinuclear region of cytoplasm [IDA]
- photoreceptor outer segment [ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA, ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID