BAIT
PRKAA1
AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- cellular response to ethanol [IEP]
- cellular response to glucose starvation [ISO, ISS]
- cellular response to hydrogen peroxide [IEP]
- cellular response to hypoxia [IEP]
- cellular response to nutrient levels [ISO, ISS]
- cellular response to organonitrogen compound [IEP]
- cold acclimation [IDA]
- fatty acid homeostasis [IDA]
- fatty acid oxidation [ISO]
- glucose homeostasis [ISO, ISS]
- glucose metabolic process [ISO]
- histone-serine phosphorylation [ISO]
- lipid biosynthetic process [ISO, ISS]
- negative regulation of TOR signaling [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of glucose import in response to insulin stimulus [IMP]
- negative regulation of lipid catabolic process [ISO, ISS]
- negative regulation of translation [NAS]
- positive regulation of autophagy [ISO, ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of fatty acid oxidation [NAS]
- positive regulation of gene expression [ISO]
- positive regulation of gluconeogenesis [NAS]
- positive regulation of glucose import [NAS]
- positive regulation of glycolytic process [IDA]
- protein heterooligomerization [IDA]
- protein phosphorylation [IDA, ISO, NAS]
- regulation of circadian rhythm [ISO, ISS]
- regulation of energy homeostasis [IDA]
- regulation of vesicle-mediated transport [IMP]
- response to UV [ISO]
- response to activity [IDA]
- response to caffeine [IDA]
- response to camptothecin [ISO]
- response to gamma radiation [ISO, ISS]
- response to hydrogen peroxide [ISO]
Gene Ontology Molecular Function- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
HSPA1B
Hsp70-2, Hsp72, Hspa2
heat shock 70kD protein 1B (mapped)
GO Process (24)
GO Function (13)
GO Component (15)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- DNA repair [ISO]
- cellular heat acclimation [ISO]
- cellular response to heat [ISO]
- mRNA catabolic process [ISO, ISS]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell death [ISO]
- negative regulation of cell growth [ISO, ISS]
- negative regulation of cell proliferation [ISO, ISS]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [ISO]
- negative regulation of inclusion body assembly [ISO]
- negative regulation of protein ubiquitination [ISO]
- neuron differentiation [IEP]
- positive regulation of erythrocyte differentiation [ISO]
- positive regulation of neuron differentiation [IMP]
- protein refolding [ISO]
- regulation of cell death [ISO]
- response to ethanol [IEP]
- response to heat [ISO]
- response to ischemia [IEP]
- response to mechanical stimulus [IEP]
- response to radiation [IEP]
- response to unfolded protein [IMP, ISO, ISS]
- telomere maintenance [ISO]
Gene Ontology Molecular Function- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [ISO]
- G-protein coupled receptor binding [ISO]
- double-stranded RNA binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein binding involved in protein folding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [ISO]
- G-protein coupled receptor binding [ISO]
- double-stranded RNA binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein binding involved in protein folding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [ISO]
Gene Ontology Cellular Component
- COP9 signalosome [ISO]
- aggresome [ISO]
- blood microparticle [ISO]
- centriole [ISO]
- cytoplasm [ISO, ISS]
- cytosol [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- inclusion body [ISO]
- nuclear speck [ISO, ISS]
- nucleus [ISO, ISS]
- perinuclear region of cytoplasm [ISO, ISS]
- ribonucleoprotein complex [ISO]
- ubiquitin ligase complex [ISO]
- vesicle [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID