BAIT
PRKAA1
AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- cellular response to ethanol [IEP]
- cellular response to glucose starvation [ISO, ISS]
- cellular response to hydrogen peroxide [IEP]
- cellular response to hypoxia [IEP]
- cellular response to nutrient levels [ISO, ISS]
- cellular response to organonitrogen compound [IEP]
- cold acclimation [IDA]
- fatty acid homeostasis [IDA]
- fatty acid oxidation [ISO]
- glucose homeostasis [ISO, ISS]
- glucose metabolic process [ISO]
- histone-serine phosphorylation [ISO]
- lipid biosynthetic process [ISO, ISS]
- negative regulation of TOR signaling [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of glucose import in response to insulin stimulus [IMP]
- negative regulation of lipid catabolic process [ISO, ISS]
- negative regulation of translation [NAS]
- positive regulation of autophagy [ISO, ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of fatty acid oxidation [NAS]
- positive regulation of gene expression [ISO]
- positive regulation of gluconeogenesis [NAS]
- positive regulation of glucose import [NAS]
- positive regulation of glycolytic process [IDA]
- protein heterooligomerization [IDA]
- protein phosphorylation [IDA, ISO, NAS]
- regulation of circadian rhythm [ISO, ISS]
- regulation of energy homeostasis [IDA]
- regulation of vesicle-mediated transport [IMP]
- response to UV [ISO]
- response to activity [IDA]
- response to caffeine [IDA]
- response to camptothecin [ISO]
- response to gamma radiation [ISO, ISS]
- response to hydrogen peroxide [ISO]
Gene Ontology Molecular Function- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
GNAO1
Gnao, RATBPGTPC
guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
GO Process (17)
GO Function (11)
GO Component (7)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IMP, ISO]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [IBA]
- aging [IDA]
- cellular process [ISO]
- dopamine receptor signaling pathway [IBA, ISO]
- forebrain development [IEP]
- locomotory behavior [ISO]
- negative regulation of calcium ion transport [IMP]
- neuron projection development [IEP]
- positive regulation of GTPase activity [IMP]
- regulation of heart contraction [ISO]
- response to cytokine [IEP]
- response to drug [IEP]
- response to hydrogen peroxide [IEP]
- response to morphine [IEP]
- response to organic cyclic compound [IEP]
- response to organonitrogen compound [IDA]
Gene Ontology Molecular Function- G-protein beta/gamma-subunit complex binding [IBA]
- G-protein coupled serotonin receptor binding [IPI]
- GDP binding [TAS]
- GTP binding [IDA, ISO]
- GTPase activating protein binding [IPI]
- GTPase activity [IDA, ISO]
- corticotropin-releasing hormone receptor 1 binding [IPI]
- mu-type opioid receptor binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- signal transducer activity [IBA]
- G-protein beta/gamma-subunit complex binding [IBA]
- G-protein coupled serotonin receptor binding [IPI]
- GDP binding [TAS]
- GTP binding [IDA, ISO]
- GTPase activating protein binding [IPI]
- GTPase activity [IDA, ISO]
- corticotropin-releasing hormone receptor 1 binding [IPI]
- mu-type opioid receptor binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- signal transducer activity [IBA]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID