BAIT
PRKAA1
AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- cellular response to ethanol [IEP]
- cellular response to glucose starvation [ISO, ISS]
- cellular response to hydrogen peroxide [IEP]
- cellular response to hypoxia [IEP]
- cellular response to nutrient levels [ISO, ISS]
- cellular response to organonitrogen compound [IEP]
- cold acclimation [IDA]
- fatty acid homeostasis [IDA]
- fatty acid oxidation [ISO]
- glucose homeostasis [ISO, ISS]
- glucose metabolic process [ISO]
- histone-serine phosphorylation [ISO]
- lipid biosynthetic process [ISO, ISS]
- negative regulation of TOR signaling [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of glucose import in response to insulin stimulus [IMP]
- negative regulation of lipid catabolic process [ISO, ISS]
- negative regulation of translation [NAS]
- positive regulation of autophagy [ISO, ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of fatty acid oxidation [NAS]
- positive regulation of gene expression [ISO]
- positive regulation of gluconeogenesis [NAS]
- positive regulation of glucose import [NAS]
- positive regulation of glycolytic process [IDA]
- protein heterooligomerization [IDA]
- protein phosphorylation [IDA, ISO, NAS]
- regulation of circadian rhythm [ISO, ISS]
- regulation of energy homeostasis [IDA]
- regulation of vesicle-mediated transport [IMP]
- response to UV [ISO]
- response to activity [IDA]
- response to caffeine [IDA]
- response to camptothecin [ISO]
- response to gamma radiation [ISO, ISS]
- response to hydrogen peroxide [ISO]
Gene Ontology Molecular Function- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
MYO5A
D, Dop, Myh12
myosin VA
GO Process (29)
GO Function (14)
GO Component (29)
Gene Ontology Biological Process
- actin filament-based movement [IDA, ISO]
- cellular response to insulin stimulus [ISO, ISS]
- developmental pigmentation [ISO]
- endoplasmic reticulum localization [ISO]
- exocytosis [ISO]
- insulin secretion [ISO]
- locomotion involved in locomotory behavior [ISO]
- long-chain fatty acid biosynthetic process [ISO]
- melanin biosynthetic process [ISO]
- melanin metabolic process [ISO]
- melanocyte differentiation [ISO]
- melanosome localization [ISO]
- melanosome transport [ISO]
- metabolic process [IDA, IMP]
- myelination [ISO]
- neuromuscular process controlling balance [ISO]
- odontogenesis [ISO]
- pigmentation [IMP, ISO]
- post-Golgi vesicle-mediated transport [ISO]
- protein localization to plasma membrane [ISO, ISS]
- regulation of Golgi organization [ISO]
- regulation of exocytosis [IMP]
- regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [ISO]
- secretory granule localization [IDA, IMP, ISO]
- synapse organization [ISO]
- synaptic transmission [ISO]
- vesicle transport along actin filament [IMP, ISO]
- vesicle-mediated transport [ISO, ISS]
- visual perception [ISO]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATP-dependent protein binding [IPI]
- Rab GTPase binding [ISO]
- SNARE binding [IPI]
- actin binding [ISO]
- calcium ion binding [ISO]
- calcium-dependent protein binding [IPI]
- calmodulin binding [ISO]
- microfilament motor activity [IDA, ISO]
- motor activity [IMP, ISO]
- poly(A) RNA binding [ISO]
- protein dimerization activity [IMP]
- protein heterodimerization activity [IDA]
- syntaxin-1 binding [IPI]
- ATP binding [IDA]
- ATP-dependent protein binding [IPI]
- Rab GTPase binding [ISO]
- SNARE binding [IPI]
- actin binding [ISO]
- calcium ion binding [ISO]
- calcium-dependent protein binding [IPI]
- calmodulin binding [ISO]
- microfilament motor activity [IDA, ISO]
- motor activity [IMP, ISO]
- poly(A) RNA binding [ISO]
- protein dimerization activity [IMP]
- protein heterodimerization activity [IDA]
- syntaxin-1 binding [IPI]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- actin filament [ISO]
- actomyosin [ISO]
- actomyosin, myosin complex part [IDA]
- axon [IDA]
- cell [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- early endosome [ISO]
- endoplasmic reticulum [ISO]
- extracellular vesicular exosome [ISO]
- filopodium tip [ISO]
- insulin-responsive compartment [ISO, ISS]
- intermediate filament [ISO]
- late endosome [ISO]
- lysosome [ISO]
- melanosome [ISO]
- membrane [ISO]
- microtubule plus-end [ISO]
- myosin complex [ISO]
- neuron projection [IDA]
- neuronal cell body [IDA, ISO]
- peroxisome [ISO]
- photoreceptor outer segment [ISO]
- recycling endosome [ISO]
- ruffle [ISO]
- secretory granule [IDA, ISO, TAS]
- synaptic vesicle [IDA]
- vesicle [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID