BAIT
PRKAA1
AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- cellular response to ethanol [IEP]
- cellular response to glucose starvation [ISO, ISS]
- cellular response to hydrogen peroxide [IEP]
- cellular response to hypoxia [IEP]
- cellular response to nutrient levels [ISO, ISS]
- cellular response to organonitrogen compound [IEP]
- cold acclimation [IDA]
- fatty acid homeostasis [IDA]
- fatty acid oxidation [ISO]
- glucose homeostasis [ISO, ISS]
- glucose metabolic process [ISO]
- histone-serine phosphorylation [ISO]
- lipid biosynthetic process [ISO, ISS]
- negative regulation of TOR signaling [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of glucose import in response to insulin stimulus [IMP]
- negative regulation of lipid catabolic process [ISO, ISS]
- negative regulation of translation [NAS]
- positive regulation of autophagy [ISO, ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of fatty acid oxidation [NAS]
- positive regulation of gene expression [ISO]
- positive regulation of gluconeogenesis [NAS]
- positive regulation of glucose import [NAS]
- positive regulation of glycolytic process [IDA]
- protein heterooligomerization [IDA]
- protein phosphorylation [IDA, ISO, NAS]
- regulation of circadian rhythm [ISO, ISS]
- regulation of energy homeostasis [IDA]
- regulation of vesicle-mediated transport [IMP]
- response to UV [ISO]
- response to activity [IDA]
- response to caffeine [IDA]
- response to camptothecin [ISO]
- response to gamma radiation [ISO, ISS]
- response to hydrogen peroxide [ISO]
Gene Ontology Molecular Function- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
- AMP-activated protein kinase activity [IDA, IMP, ISO]
- ATP binding [IDA]
- chromatin binding [ISO, ISS]
- eukaryotic elongation factor-2 kinase activator activity [NAS]
- histone serine kinase activity [ISO, ISS]
- kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein serine/threonine kinase activity [IDA]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
ATP2A2
Serca2, SercaII
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
GO Process (20)
GO Function (9)
GO Component (13)
Gene Ontology Biological Process
- ATP catabolic process [IDA, ISO]
- ER-nucleus signaling pathway [ISO]
- calcium ion import into sarcoplasmic reticulum [IDA]
- calcium ion transmembrane transport [IDA, ISO]
- calcium ion transport [IDA]
- calcium ion transport from cytosol to endoplasmic reticulum [ISO]
- cellular calcium ion homeostasis [ISO]
- endoplasmic reticulum calcium ion homeostasis [ISO]
- negative regulation of heart contraction [ISO]
- positive regulation of endoplasmic reticulum calcium ion concentration [ISO]
- regulation of calcium ion-dependent exocytosis of neurotransmitter [ISO]
- regulation of cardiac muscle cell action potential involved in regulation of contraction [IDA]
- regulation of cardiac muscle cell membrane potential [IDA]
- regulation of cardiac muscle contraction by calcium ion signaling [IDA, ISO]
- regulation of muscle contraction [TAS]
- regulation of the force of heart contraction [ISO]
- relaxation of cardiac muscle [IDA, ISO]
- response to peptide hormone [IDA]
- sarcoplasmic reticulum calcium ion transport [IDA]
- transition between fast and slow fiber [ISO]
Gene Ontology Molecular Function- ATP binding [IDA]
- S100 protein binding [ISO]
- calcium ion binding [IDA, ISO]
- calcium-transporting ATPase activity [IDA, ISO]
- calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential [IDA]
- enzyme binding [ISO]
- lutropin-choriogonadotropic hormone receptor binding [IPI]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- ATP binding [IDA]
- S100 protein binding [ISO]
- calcium ion binding [IDA, ISO]
- calcium-transporting ATPase activity [IDA, ISO]
- calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential [IDA]
- enzyme binding [ISO]
- lutropin-choriogonadotropic hormone receptor binding [IPI]
- protein C-terminus binding [ISO]
- protein binding [IPI]
Gene Ontology Cellular Component
- calcium ion-transporting ATPase complex [ISO]
- cell [ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [ISO]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- intercalated disc [ISO]
- longitudinal sarcoplasmic reticulum [ISO]
- membrane [ISO]
- perinuclear region of cytoplasm [IDA]
- protein complex [IDA]
- sarcoplasmic reticulum [IDA, ISO, TAS]
- sarcoplasmic reticulum membrane [IC]
- vesicle membrane [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]
Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]
Throughput
- High Throughput
Curated By
- BioGRID