BAIT

PRKAA1

AMPKalpha1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (36)
GO Function (10)
GO Component (6)
Rattus norvegicus
PREY

RPL10A

ribosomal protein L10A
GO Process (0)
GO Function (1)
GO Component (8)
Rattus norvegicus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.

Moon S, Han D, Kim Y, Jin J, Ho WK, Kim Y

The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in ... [more]

Sci Rep Mar. 15, 2014; 4(0);4376 [Pubmed: 24625528]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PRKAA1 RPL10A
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

High-BioGRID
2546321

Curated By

  • BioGRID